DETECTION OF STAPHYLOCOCCUS AUREUS CLASSIC ENTEROTOXIN GENES IN SOME MEAT PRODUCTS USING MULTIPLEX PCR

Abstract

A total of 200 meat product samples of beef burger, sausage, minced meat and luncheon were randomly collected from different shops and markets in El-Gharbia and Kafr-El-Sheikh governorates. The samples from 3 brands A, B and C were examined for the presence S. aureus using classical bacteriological and molecular methods. The obtained results revealed thatthe 2, 1 and 4 samples of beef burger, sausage and minced meat respectively, were positive for S. aureus by PCR. Additionally multiplex PCR was used for detection of five S. aureus virulence classic enterotoxin genes (sea, seb, sec, sed and see). The obtained results revealed thatthe beef burger samples harbored (1) seb gene; meanwhile sausage sample harbored (1) sea gene and minced meat samples harbored (2) sea, (1) sec and (1) sed genes. This study offers the basis for further phenotypic and molecular characterization S. aureus isolated from these products to guarantee safe consumption of meat products.