Zinc Single-Atom Nanozyme As Carbonic Anhydrase Mimic for CO₂ Capture and Conversion

Abstract

Single-atom nanozymes (SANs) are a class of nanozymes with metal centers that mimic the structure of metalloenzymes. Herein, we report the synthesis of Zn-N-C SAN, which mimics the action of the natural carbonic anhydrase enzyme. The two-step annealing technique led to a metal content of more than 18 wt %. Since the metal centers act as active sites, this high metal loading resulted in superior catalytic activity. Zn-SAN showed a CO₂ uptake of 2.3 mmol/g and a final conversion of CO₂ to bicarbonate of more than 91%. CO₂ was converted via a biomimetic process by allowing its adsorption by the catalyst, followed by the addition of the catalyst to HEPES buffer (pH = 8) to start the CO₂ conversion into HCO₃ ^-. Afterward, CaCl₂ was added to form a white CaCO₃ precipitate, which was then filtered, dried, and weighed. Active carbon and MCM-41 were used as controls under the same reaction conditions. According to the findings, the CO₂ sequestration capacity was 42 mg of CaCO₃/mg of Zn-SAN. Some amino acids (AAs) with binding affinity for Zn were able to suppress the enzymatic activity of Zn-SAN by blocking the active metal centers. This strategy was used for the detection of His, Cys, Glu, and Asp with detection limits of 0.011, 0.031, 0.029, and 0.062 μM, respectively, and hence was utilized for quantifying these AAs in commercial dietary supplements.