Using Sexed Semen to Produce Homo-Sexual Twins from Dairy Friesian Cows Under Egyptian Condition

Abstract

The presented study was conducted to investigated the impact of vaginal and uterine pH on the efficiency of fertilization with sexed semen of dairy Friesian cows. Thirty Friesian anestrus cows were used and divided into three groups (10 each). The uteri of cows in the two groups was washing with pH buffer solution, being 6 for 1st group, and 8 for 2nd group, while the 3rd group not washing and kept as a control group. All groups were subjected to a Cosynch+progesterone protocol to induce estrus, and ultrasound scanning, and blood samples were taken at various intervals. This study results found that adjusting the pH level had no significant effect on ovarian follicle development, but it have a significant effect on the rate and growth of dominant follicles in the acidic groups. Finally, adjusting the pH of the vagina and uterus during insemination to 6 for X- sexed semen and 8 for Y- sexed semen increases semen efficiency, and resulting in identical twins of 60% for AC and 40% for AK compared to 10% for conventional semen.