Therapeutic Potential of Hyaluronic acid hydrogel /Bone marrow stem cell-conditioned medium on Articulating Surfaces Associated with Induced Arthritis in Rabbit’s TMJ

Abstract

Aim: To assess hyaluronic acid hydrogel /bone marrow stem cell-conditioned medium (MSC-CM with HA hydrogel) on articulating surfaces associated with induced arthritis in rabbit’s TMJ. Subjects and methods: , fifty rabbits were employed. A technique to induce arthritis was performed on forty rabbits using a double IA injection of CFA. There were made five groups of rabbits. 10 rabbits in Group I (the normal group) and 10 in Group II (the arthritic group) did not receive any treatment, respectively. Ten animals each got a bilateral IA injection of 0.2 ml of Group III hyaluronic acid hydrogel.10 rabbits each got a bilateral IA injection of 0.2 ml of Group IV and bone marrow stem cell condition media.10 rabbits were given bilateral IA injections of 0.2ml of a combination therapy (hyaluronic acid hydrogel and bone marrow stem cell condition medium (Group V). After the fourth week of injection-based therapy, all rabbits will be anesthetized then euthanized. Dissection, decalcification, and preparation Aim: To assess hyaluronic acid hydrogel /bone marrow stem cell-conditioned medium (MSC-CM with HA hydrogel) on articulating surfaces associated with induced arthritis in rabbit’s TMJ. Subjects and methods: , fifty rabbits were employed. A technique to induce arthritis was performed on forty rabbits using a double IA injection of CFA. There were made five groups of rabbits. 10 rabbits in Group I (the normal group) and 10 in Group II (the arthritic group) did not receive any treatment, respectively. Ten animals each got a bilateral IA injection of 0.2 ml of Group III hyaluronic acid hydrogel.10 rabbits each got a bilateral IA injection of 0.2 ml of Group IV and bone marrow stem cell condition media.10 rabbits were given bilateral IA injections of 0.2ml of a combination therapy (hyaluronic acid hydrogel and bone marrow stem cell condition medium (Group V). After the fourth week of injection-based therapy, all rabbits will be anesthetized then euthanized. Dissection, decalcification, and preparation of the mandible for Masson’s Tri-chrome staining. Results: Those different treatment modalities responded to osteoarthritis in different ways, with MSC-CM with HA hydrogel being regarded as the most effective treatment of induced temporomandibular joint osteoarthritis among the tested treatment modalities. Conclusion: It was possible to conclude, within the limitations of the current study, MSC-CM with HA hydrogel being regarded as the most effective treatment of induced temporomandibular joint osteoarthritis