The influence of lncRNA expression dysregulation on predicting celiac diseases among patients with Hashimoto's thyroiditis

Abstract

Aim: the study aimed to investigate lncRNA IFNG-AS1 in Hashimoto’s thyroiditis (HT) and to evaluate its predictive and diagnostic values of Celiac disease (CD) among patients with HT. Patients and Methods One hundred voluntary subjects: 50 healthy controls and 50 patients with HT;39 without CD and 11 with CD. Routine labs, including anti-TG, anti-TPO, and anti-TTG-IgA, investigated all subjects. lncRNA IFNG-AS1 expression was analyzed using Quantitative real-time RT-PCR.ResultsPatients with CD had significantly overexpressed lncRNA IFNG-AS1(4.17±0.618) compared to patients without CD (1.92±0.52) and control group (0.98±0.21), p ˂0.001*. It correlated considerably with intestinal and extra-intestinal manifestations and laboratory tests, including anti-TG, anti-TPO, and TTG IgA. Multivariate regression confirmed that the only variables independently associated with lncRNA IFNG-AS1 in the prediction of CD among studied parameters were anti-TG, anti-TPO, and TTG IgA. We found high sensitivity and specificity of 96% and 94% among HT, respectively. Concerning CD, the predictive power of IFNG-AS1 had sensitivity and specificity of 63.3% and 53%, respectively.Conclusion: lncRNA IFNG-AS1 level was overexpressed in patients with HT, particularly those with CD, and correlated to clinical and laboratory parameters. Accordingly, it may be a promising predictive biomarker for HT and CD.