The Effect of Nano-NeoMTA2 and Nanohydroxyapatite on the Human Dental Pulp Stem Cells Odontogenic Differentiation and Proliferation

Abstract

OBJECTIVE: To evaluate the effect of Nano-NeoMTA2 on human dental pulp stem cells (hDPSCs) differentiation and proliferation potential compared to nanohydroxyapatite. METHODS: After the preparation and characterization of the nanoparticles of NeoMTA2 and hydroxyapatite, the nanoparticles were mixed prior to application. The mixed nanoparticles were added to hDPSCs harvested from third molar teeth immediately upon extraction, in addition to a negative and positive control group. Alkaline phosphatase and dentin matrix protein were used as measures for the odontogenic differentiation potential of the hDPSCs. Human DPSC count and viability of Trypan Blue and [3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide] assay were used as a measure of the proliferation potential. RESULTS: Nano-NeoMTA2 demonstrated significantly greater induction of hDPSC proliferation and odontogenic differentiation than nanohydroxyapatite. CONCLUSIONS: Both tested nano-biomaterials induce DPSC odontogenic differentiation and proliferation. Nano-NeoMTA2 stimulates odontogenic differentiation and proliferation of hDPSCs and warrants further investigation as a potential scaffold or direct pulp-capping material.