Synthesis and characterization of self-assembly nanocomposite of ginger extract/cellulose of cotton supported by zinc oxide nanoparticle for destroying the <i>Cryptosporidium parvum</i> oocysts

Abstract

The present study describes how increasing efficacy without affecting cell viability, using cellulose nanocrystals (CNC) that consider a self-assemble compound with using the ginger extract (GE) for this purpose can increase the effectiveness of treating cryptosporidiosis (C. parvum). A nanocomposite (NCP) can be effectively synthesized using chemical precipitation techniques without the use of temperature, and it can also be successfully characterized using a variety of ways to show that the NCP was naturally formed. According to a UV-visible spectroscopic device's calculation of the EG extract's loading efficiency on the CMC surface, the loading efficiency is 58%. The liver cells of mice (BNL) are used to test the cytotoxicity of NCP at various concentrations using the rapid screening cytotoxicity technique (SRB). After 96 hours, the sporulation percentage with the maximum dose of 7 mg/mL was reported at 45.97% ± 0.65%, 43.97% ± 0.65, 31.3% ± 0.7 for GE, CNC@GE, and CNC@GE.ZnO NCP respectively. After 96 hours, the distortion percentage with the maximum dose of 7 mg/mL was reported at 53.97% ± 0.65, 55.97% ± 0.65, 68.63% ± 0.65 for GE, CNC@GE, and CNC@GE.ZnO NCP respectively. The findings support the use of modified NCP as a cryptosporidiosis treatment substitute, but further clinical testing is required.