article · Horticulturae
Salinity stress limits crop growth, but combining melatonin and putrescine can significantly improve resilience in snap bean seedlings. Treating salt-stressed seedlings with both compounds together increases shoot fresh and dry weight, photosynthetic pigments including chlorophyll and carotenoids, soluble sugars, proline, potassium, and calcium. The combined treatment also reinforces cell membrane stability and elevates protective catalase and peroxidase enzyme activities. Concurrently, it lowers harmful sodium accumulation, the sodium-to-potassium ratio, and oxidative damage markers such as hydrogen peroxide and malondialdehyde. The dual treatment also suppresses polyamine oxidase and diamine oxidase activities, restricting polyamine degradation. While individual melatonin application boosts relative water content and certain antioxidant enzymes, the combined treatment delivers superior overall salinity tolerance and tissue protection in young bean plants.
Soil salinity presents a growing barrier to crop productivity and food security worldwide. Understanding how chemical treatments such as melatonin and putrescine interact helps identify effective methods for protecting sensitive vegetable crops from salt damage. This insight could guide better crop management strategies to preserve seedling growth and vitality under saline growing conditions.
The findings could inform the development of chemical treatments, seed coatings, or biostimulant formulations to safeguard legume crops in salt-affected soils. Potential end users include agrochemical developers, commercial seedling nurseries, and horticulture growers dealing with saline water or land. This research is at an early experimental stage, having been tested on snap bean seedlings in controlled conditions, and requires field-scale validation before commercial adoption.
AI-generated from the published abstract. Always read the original work before citing.
While the individual influences of melatonin (MT) and polyamines (PAs) have been widely studied under various abiotic stresses, little is known about their interaction under salinity stress. In the present study, salt stress applied by 50 mM of sodium chloride (NaCl) on snap bean seedlings has been supplemented with 20 μM of MT and/or 100 μM of putrescine (Put) (individually and in combination). The results indicated that under salinity stress, the combination of MT + Put achieved the highest significant increase in shoot fresh and dry weight, chlorophyll (Chl a), Chl a + b, carotenoids, total soluble sugars, proline, K, Ca, and cell membrane stability index (CMSI), as well as catalase (CAT) and peroxidase (POX) activities. This improvement was associated with an obvious decrease in Na, Na/K ratio, and oxidative damage as indicated by reducing leaf contents of methylglyoxal (MG), hydrogen peroxide (H2O2), and the rate of lipid peroxidation (malondialdehyde; MDA). Moreover, the combination of MT + Put demonstrated a significant decrease in the activities of diamine oxidase (DAO) and polyamine oxidase (PAO) leading to the reduction of the rate of polyamine oxidation. Meanwhile, MT applied individually gave the highest significant increase in leaf relative water content (RWC), Chl b, superoxide dismutase (SOD), and ascorbate peroxidase (APX). Conclusively, the combination treatment of MT + Put could decrease the degradation of polyamines and enhance tolerance to salinity stress in snap bean seedlings.
This page summarises published work. The authoritative version sits with the publisher.
DOI: 10.3390/horticulturae9020285
Is something wrong with this record? Report it or request removal.
Discussion
Have you built on this work, tried to replicate it, or seen it applied in practice? Share what you know. Verified researchers and MARATTO™ domain experts can open a discussion, and any member can reply. Contributions are reviewed before they appear.
No discussion yet. Open the first thread.
New to MARATTO™? Create a free account.
