Surface Plasmon Resonance‐based Biosensing Towards the Detection of Multidrug‐Resistant Tuberculosis

Abstract

Current diagnostic tools for multidrug‐resistant tuberculosis (MDR‐TB) are molecular assay‐based and have challenges associated with labor‐intensive workflows, complex laboratory infrastructures, and limited mutation coverage. This highlights the need for alternative techniques that can be used as diagnostic tools for MDR‐TB. In this study, we demonstrated the use of an surface plasmon resonance (SPR)‐based biosensor chip for the detection of selected genes ( InhA , KatG , and RpoB ) within the MDR‐TB genome using single‐stranded deoxyribonucleic acids (ssDNA) targets and thiolated probes. The probes were successfully functionalized to AuNPs and confirmed using UV–vis and DLS. On SPR‐based detection, the hybridization of the selected probes to complementary and non‐complementary targets induced changes in the resonance angles. The hybridization of the selected probes to the targets was observed at resonance angles of 46.85, 46.77, 45.84, and 46.91° for the IS6110 , InhA , KatG , and RpoB genes, respectively. In contrast, the unhybridized probe and the non‐complementary targets exhibited resonance angles of 46.33, 46.05, 45.53, and 45.85° for the IS6110 , InhA , KatG , and RpoB genes, respectively. The data showed that SPR‐based biosensing can be refined and considered as an alternative approach to detect and differentiate between different ssDNA targets using thiolated probes as biorecognition elements for MDR‐TB detection.