Study of the Role of Migration Inducting Gene 7 as a Molecular Marker for Early Detection of Endometrial Carcinoma Micrometastasis.

Abstract

Background and objectives: Endometrial carcinoma diagnosed early by abnormal uterine bleeding especially in postmenopausal women. Migration-Inducting Gene 7 (MIG-7) is a cysteine-rich protein first identified in endometrial cancer cells upon hepatocyte growth factor (HGF) treatment.Subjects and methods: This case-control study was carried out in Mansoura University, Egypt on a total of 75 patients with 25 age-matched healthy female volunteers as control group and 50 patients classified according to standard criteria based on data of International Federation of Gynecology and Obstetrics (FIGO) into two groups (non-metastatic endometrial carcinoma and metastatic endometrial carcinoma). Total RNA extracted from peripheral blood, cDNA synthesized from RNA and Real-time PCR done for quantification of MIG7.Results: Comparison between the control group, patient with non-metastatic endometrial carcinoma and patient with metastatic endometrial carcinoma with regard to MIG7 gene expression reveal highly significant differences ((0.69±0.21), (1.38±0.39) and (1.97±0.45)) respectively, P-values (all˂ 0.001). AUC values for MIG7 equal 0.945 while MIG7 show sensitivity percentage of 88% and 88% specificity percent.In the univariable analysis MIG7 gives an odds ratio (ORs) 1.077 at p-values of 0.002 while in the multivariable analysis demonstrate elevated ORs, p-values (0.024).