Spectrofluorimetric quantification of terconazole in pharmaceutical creams with comprehensive sustainability evaluation

Abstract

Terconazole is an effective antifungal drug with a broad spectrum of activity against many types of fungi. In the study, a spectrofluorimetric method was developed for the quantification of terconazole. Eosin Y, an efficient fluorescence probe, was used in the developed work. The developed method is based on forming a non-fluorescent association complex between terconazole and eosin Y, which quenches eosin Y's inherent fluorescence intensity at both excitation and emission spectra. This quenching effect is highly correlated with the concentration of terconazole, with a linear range of 0.15-1.2 µg/mL. Furthermore, Stern-Volmer analysis was carried out to study the quenching fluorescence strength of eosin Y by terconazole. Moreover, the developed method exhibits an LOD of 0.041 µg/mL and a LOQ of 0.125 µg/mL. Moreover, the recommended method was verified in accordance with ICH guidelines. In addition, with respectable recovery values, the established procedures were successfully applied to the analysis of terconazole in vaginal cream dosage forms. Furthermore, the greenness of the entire proposed project was assessed using the following assessment tools: MoGAPI, Complex MoGAPI, MoGSA, BAGI, and RAPI. The green profiles provided outstanding evidence of the greenness and environmental friendliness of the proposed approach.