Pseudomonas putida Endophytic Bacterium Isolated from Typha domingensis as Ecofriendly Tool to Remove Heavy Metal from Environment

Abstract

Heavy metal contamination has posed a serious threat to environmental stability because of its extreme toxicity and lack of biodegradability. Microbial remediation shown the most efficient and ecofriendly solution. The current study focuses on examination of Pseudomonas putida for its ability to tolerate heavy metals and its growth promoting activities. Pseudomonas putida was characterized according to morphology, biochemical, removal efficiency, and growth promoting. It was identified as Pseudomonas putida (PP077069). It showed a potential for the remediation of metal-containing wastewater with capacity to reduce the ecotoxicity of the effluent. It was Fe-, Cr-, and Cu-tolerant with MIC values (5, 7, and 3.5 mgl-1). The highest removal efficiency was observed after five days (11.54, 18.62, and 10.04%), with metal uptake capacities (0.58, 1.3, and 0.35 mgl-1), respectively. It is concluded that seed priming can be regarded as a promising approach for increasing the resistance of wheat seedlings. Pseudomonas putida had abilities to phosphate solubilization, siderophore, HCN production and IAA synthesis. Priming with P. putida had a significant stimulatory effect on the biochemical and physiological performance of wheat seedlings compared to unprimed seeds.