Protective Effect of Chlorella vulgaris and Spirulina platensis against ThioacetamideInduced Hepatorenal Toxicity in Male Rats

Abstract

The present inquiry evaluated the possible pharmacological effects of Chlorella vulgaris(CV) and Spirulina platensis (SP) with special reference to their hepatorenal protectiveeffect as well as their antioxidant activities against thioacetamide (TAA) acute toxicity.Thirty-six male Wister albino rats, weighing from 100 to 120 gm were applied in thepresent study. Rats were randomly allocated into 6groups each of 6 animals. Group I wasmaintained as the control healthy group. It was administrated distilled water orally for onemonth and normal saline at a dose of 1 ml IP. On the last two days of the experiment. Theother groups were allocated into 5 groups including A, B, C, D and E. Group A was used as acontrol intoxicated with thioacetamide at a dose of 300 mg/ kg b.wt. IP for two days with24 hrs intervals before the end of the experiment. Group B was stated as a standardprotected group and it was treated with silymarin at a dose of 100 mg/kg b.wt. orally forone month. Group C was given Chlorella vulgaris orally at a quantity of 400 mg/kg b.wt.daily, for one month. Group D was given Spirulina platensis at a dose of 400 mg /kg b.wt.Orally daily for one month. Group E was given CV &SP at doses of 400 mg /kg b.wt eachorally daily for one month. Groups B, C, D, and E were intoxicated with thioacetamide(TAA), at an amount of 300mg/kg b.wt. IP for two days with 24 hrs intervals at the end ofthe month. Toxicity of rats with thioacetamide substantially elevated the levels of alaninetransferase and aspartate aminotransferase, serum urea, creatinine, and uric acid inaddition to increased malon-di-aldehyde concentration. However, it significantly decreasedtotal proteins, and total antioxidant capacity concentrations and decreased bloodparameters. As well as it induced histopathological alterations in hepatic and renal tissuedesigns (delete). On the other hand, oral administration of Chlorella vulgaris and Spirulinaplatensis ameliorated TAA-induced biochemical, pathological, and histopathologicalchanges in hepatic tissues and renal tissues. This study stated that these algae attenuatethioacetamide and protect against hepatorenal toxicity, via their antioxidant properties.