Preliminary In Vitro Tests to Demonstrate Various Formulations' Effect on the Stability of the Moroccan Trichoderma asperellum-Based Product's Biology

Abstract

The selection of the T asperellum-based product required several in vitro tests in order to preserve its efficacy during the formulation process, while maintaining the viability of the conidia and safeguarding their antagonistic properties. The addition of talc to all of the compounds added to the formulation of T asperellum's conidia, Moroccan strain isolated from compost, preserved their viability, stability and antagonistic power during the entire duration under storage conditions. P1 and P2's conidia germination percentages while stored for 38 weeks at room temperature were 0% - 12% and at 4°C were 31% - 46.33% respectively. The antagonistic effect of P1 and P2 against V dahliae by direct contact is estimated by the T asperellum colonization percentages, the mycelial growth of V dahliae's inhibition percentages, the mycelial growth of V dahliae's inhibition percentages and the mycelial inhibition percentages by antibiosis are respectively 0 - 79.58%, 0 - 39.24% and 3.25 - 39.84% at room temperature and 80.22 - 81.49%, 39.83 - 44.99% and 42.27 - 43.09% at 4°C.