Potential Curative Effects of Taurine and Curcumin on Rats Liver with Pre-eclampsia Induced by Doxorubicin

Abstract

This study was conducted to investigate the effects of taurine or/and curcumin supplementations on the liver of pre-eclamptic rats. Pre-eclampsia was induced in pregnant rats by 10mg adriamycin /kg. b.wt. intraperitoneal (tri-weekly) for 2 weeks. The pregnant rats were dissected on the 20th day of gestation. The rats were examined for morphological changes, biochemical analysis and DNA damage evaluation. Significant alterations were recorded in the number of implantation and resorption sites, live fetuses, activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), γ-glutamyl-transferase (GGT) as well as concentrations of albumin and total protein associated with disturbance in the levels of haptoglobin (HPG), liver-fatty acid binding protein (L-FABP), Total antioxidant status (TAS) and total oxidant status (TOS) in pre-eclampsia groups compared with their corresponding control ones. Also, external morphological examination of fetuses exhibited malformed and resorbed fetuses in toxemic group. Comet assay from liver tissues of all pregnant mother rats were measured which showed high percentage of DNA damage 58.48, 36.27, 52.05 and 25.14% in V, VI, VII and VIII toxemic rat groups respectively than control group 5.93, 5, 3.77, 4.53% in I, II, III and IV rat groups respectively. Remarkable corrections had occurred in all previously studied parameters after being treated orally with 500mg taurine /kg. b.wt/day and/or 500mg curcumin /kg. b.wt/day for 2 weeks. Co-administration of taurine and curcumin to the pre-eclamptic rats’ group caused the maximum correction effects on all studied parameters due to modulation of free radical scavenger as well as cell membrane stabilizer through reduction of lipid peroxidation production and enhancement of cellular antioxidant enzymes system”.