Polyphenolic extract from Entada africana Guill. Pers is a potential antioxidant and anti-Tumor Necrosis Factor alpha (TNFα) ingredient

Abstract

Entada africana Polyphenolic Extract (PEE) revealed higher contents of total polyphenols (264.91±1.51mg EAG/g of extract), flavonoids (14.26 ±0.26mg EQ.g of extract) and flavonols (2.1±0.03mg EQ/g of extract). Concentration of Scavenging 50% (CS50) of hydroxyl radical (HOo) radicals with PEE (20.65 ± 0.35 μg/mL) compared to that of vitamin C (7.90 ± 0.00 μg/mL) showed a significant difference at p< 0.05 for HOo scavenging activities. The PEE extract inhibited lipidic peroxidation with IC50 of 9.70 ± 0.21 μg/mL but not significantly different from vitamin C (8.70 ± 0.14 µg/mL). PEE extract have inhibited the production of TNFα with IC50 (71.05 ± 1.20 µg/mL) significantly (p< 0.05) when compared to 25% of methylene chloride fraction of Khaya grandifoliola (32.10 ± 1.13 µg/mL) considered as a standard. For the activation of erythrocytes plasma membrane redox system (PMRS), PEE had an Effective Concentration 50% (EC50) of 8.4 ± 0.00 µg/mL relatively less than that of quercetine 4.6 ± 0.00 µg/mL. Moreover, PEE inhibits proteins oxidation in a dose-concentration manner with an IC50 of 14.60 ± 1.13 µg/mL significantly different (p< 0.05) from that of quercetin (4.60 ± 0.00 µg/mL) considered as a standard. Altogether, PEE could be used as potential antioxidant and anti-Tumor Necrosis Factor alpha (TNFα) ingredient for food and nutrition. Keywords: Entada africana, polyphenols, antioxidant, anti-TNFα, erythrocytes PMRS