Point-of-care colorimetric biosensing using 2D Co-MOFs nanozymes for sensitive detection of glucose and hydrogen peroxide

Abstract

Abstract The development of inorganic nanomaterial-based enzyme mimics has emerged as a promising strategy to overcome the high cost and limited stability of natural enzymes. Metal–organic frameworks (MOFs) are particularly appealing for biomimetic catalysis due to their high surface area, adjustable composition, and accessible functional sites. In this study, we present a point-of-care (POC) colorimetric biosensor that employs two-dimensional cobalt-based MOFs (2D Co-MOFs) as peroxidase-like catalysts for the detection of glucose and hydrogen peroxide (H₂O₂). The use of a 2D MOF structure provides improved exposure of active sites compared to bulk MOFs, contributing to reliable and efficient catalytic performance. The sensor demonstrates a linear detection range for H₂O₂ from 5.0 μM to 200.0 μM with a detection limit of 4.60 μM, and enables glucose detection within a linear range of 27.8 μM to 666.0 μM. The nanozyme was characterized using SEM,XRD and FTIR spectroscopy. The suggested platform is ideal for point-of-care application due to its sensitivity, low detection limits, and simple operation. The results demonstrate that 2D Co-MOF nanosheets can function as efficient enzyme mimics for biomolecule detection and could potentially help in the advancement of practical diagnostic sensor development.