Phenotypic and molecular characterization of methicillin-resistant Staphylococcus aureus (MRSA) circulating into environments in Ouagadougou, Burkina Faso.

Abstract

<title>Abstract</title> Meticillin-resistant <italic>Staphylococcus aureus</italic> (MRSA) diagnostic techniques are not very easy in rattan practices in Burkina Faso and West African. This is main difficulty of good MRSA surveillance in this region. Thus, to consolidate MRSA data in locality that, 33 strains of <italic>S. aureus</italic> were tested for susceptibility to cefoxitin according using standard method. In addition, the genetic determinants including <italic>mecA</italic> gene and <italic>SCCmec</italic> cassettes were searched using conventional PCR technic to same strains. These protocols made it possible to obtain prevalence’s of 42.42% (14/33) and 18.18% (6/33) respectively of <italic>S. aureus</italic> resistant to cefoxitin and positive in Chrom MRSA ID® agar medium. From 33 <italic>S. aureus</italic> studied, 6 strains (18.18%) and 7 strains (21.21%) all of human origin were respectively positive for <italic>mecA</italic> resistance gene and <italic>SCCmec</italic> IVa cassette. This study brought circulating MRSA in the city of Ouagadougou. These MRSA harbor <italic>mecA</italic> resistance genes and <italic>SCCmec</italic> IVa cassettes.