P-508 EFFECTS OF PESTICIDE EXPOSURE ON OXIDATIVE STRESS AND DNA METHYLATION BIOMARKERS IN MOROCCAN FARMERS FROM THE PAPOE STUDY

Abstract

Abstract Introduction In Morocco, due to the lack of education and the presence of a counterfeit and illegal market, pesticides constitute a major problem to be addressed by occupational and environmental health. Growing evidence suggests a link between pesticide exposure and epigenetic changes, including DNA methylation variations. In this context, we aim to examine comprehensively whether multi-residue exposure to commonly used pesticides could induce epigenetic alterations. Methods The PaPOE project includes a cross-sectional study assessing the occupational exposure among 322 farmworkers and pesticide appliers in Fez-Meknes Region (Morocco). Survey questionnaires have been conducted between 2018 and 2022 to assess pesticide exposures and work practices of the farmers. Urine and blood were collected. The biomonitoring approach using LC-MS and GC-MS targeted the profiling of 28 pesticides and their metabolites in urine. Oxidative stress biomarkers include GSH and 8-OHdG. Global DNA methylation is performed using LC-MS in blood. Results Overall, knowledge was significantly associated with education level and previous training on pesticide use, while health risks were significantly associated with the use of PPE. Pesticides were detected in 77% of samples, with mainly organophosphates and organochlorines. Glutathione and 8-OHdG were significantly associated with pesticide exposure. Global DNA methylation status is altered in the pesticide-exposed population compared to the non or less-exposed group. Conclusion Our study paves the way for a biomonitoring system in Morocco to assess environmental and occupational exposures and to provide insight into the long-term course and potential health consequences of pesticide exposure.