Oxidative Stress Long-term effects of Lipopolysaccharide on Hippocampus and Prefrontal cortex in Male and Female Wistar Rats

Abstract

Lipopolysaccharides (LPS) are lipoglycans large molecules consisting of a polysaccharide and a lipid, including in the structure of Gram-negative bacteria outer membranes. These moleculescausedysfunction of severaltissues and organslike brain. How itsinfluencethe cognitive functionis still unknown. In our study, we assessthe oxidative stress prolongedeffects of Lipopolysaccharide in tissues of prefrontal cortex and Hippocampus in Wistar Ratsat different stages of their life, adolescent, young adult and adult. 24 rats were randomly divided into four groups; 1) LPS-male (n = 3), (2) Control male (n = 3), (3) LPS-female (n = 3), and (4) Control female (n = 3). After 2, 3 and 10 months, we sacrificed rats and isolate their encephala. The levels of thiobarbituric acid reacting substances (TBARS) and nitric oxide (NO) were evaluated in the hippocampus and prefrontal cortex by calorimetric method; Griess reaction for NO, TBARS for lipid peroxidation. Differences between groups (control/LPS-males/LPS-females) were assessed using one-way ANOVA. The results reveal an increased antioxidant enzyme activities in adolescent and adult LPS-male rat brains(p<0.05); However, LPS administration did not affect antioxidant activity in nervous structures of brain in LPS-female rats. LPS administration can be responsible for damages in adolescent male rat brains, the effect of LPS in oxidative stress caused by LPS differs between males and females.