Optimized QIAGEN DNeasy Plant Pro Kit Protocol for Fungi gDNA Extraction v2

Abstract

This protocol describe the optimisation of a commercial kit "DNeasy Plant Pro Kit, QIAGEN" reserved to gDNA extraction from plant. This protocol provides clear, straightforward, and transparent mandatory steps to ensure reproducibility and repeatability in the manual extraction of genomic DNA (gDNA) from mycelium liquid cultures or mushroom tissues. It has been successfully tested across various species belonging to the Basidiomycota and Ascomycota fungi phyla. To evaluate gDNA quality, multiple validation steps were applied to the extracted samples. To evaluate gDNA quality, multiple validation steps were applied to the extracted samples. The protocol was effectively employed by Cyrine et al. 2026 (https://doi.org/10.3390/jof12050299) for gDNA extraction from diverse fungal samples, supporting downstream genomic analyses.