New Pyrazole‐Based Tetrazole Derivatives: Synthesis, Characterization, and Their Vasorelaxant and α‐Amylase Inhibition Activities

Abstract

ABSTRACT A series of new linear pyrazole‐based tetrazole derivatives 1 – 10 were synthesized and characterized. The structures of the intermediate compounds were confirmed using 1 H and 13 C NMR spectroscopy, as well as high‐resolution mass spectrometry (HRMS). These derivatives were synthesized through a straightforward equimolar condensation between pyrazole and tetrazole precursors. The vasorelaxant activity of these compounds was assessed by determining their percentage inhibition, E (%), which ranged from 26% to 67%, with compound 4 exhibiting the highest activity. Additionally, their anti‐diabetic potential was evaluated by determining the IC 50 values for α‐amylase enzyme inhibition. Notably, compounds 2 and 6 demonstrated a comparable activity to the positive control acarbose. These experimental findings were further supported by molecular docking studies.