article · Membranes
Current treatments often struggle to accelerate the healing of infected wounds, driving demand for novel therapeutic options. This research evaluated electrospun nanofibers made from nano-chitosan, eucalyptus oil, and cellulose acetate as potential wound dressings. Encapsulating eucalyptus oil within chitosan nanoparticles substantially improved its antimicrobial performance, producing a three-fold increase in bacterial inhibition against Staphylococcus aureus compared to the free oil. Electrospinning produced uniform, thin nanofibers measuring 98 nanometres across. Laboratory evaluations on human normal melanocytes demonstrated acceptable biocompatibility, maintaining 80 percent cell viability at tested concentrations. Furthermore, both laboratory and animal assessments established that the composite nanofibers were safe and accelerated wound repair. This accelerated healing was mediated by stimulating the expression of transforming growth factor beta alongside types I and III collagen. Together, the findings demonstrate the viability of these composite nanofibers for managing infected wounds.
Infected skin wounds present substantial clinical challenges because conventional treatments can fail to prevent bacterial growth while actively promoting tissue regeneration. Developing biocompatible, natural-derived dressings that combine strong antimicrobial action with accelerated tissue repair can improve patient outcomes. This approach offers a potential dual-action intervention capable of controlling bacterial infection and supporting essential structural healing processes in damaged skin.
The technology serves as a wound dressing for treating infected skin injuries, relevant to medical device manufacturers, wound-care specialists, and clinical healthcare providers. With successful testing in both laboratory cell cultures and animal models, the research stands at an applied stage of pre-clinical development. Moving toward real-world commercialisation will require larger pre-clinical trials, formal safety validation, manufacturing scale-up, and regulatory clearance for clinical use.
AI-generated from the published abstract. Always read the original work before citing.
Accelerated wound healing in infected skin is still one of the areas where current therapeutic tactics fall short, which highlights the critical necessity for the exploration of new therapeutic approaches. The present study aimed to encapsulate Eucalyptus oil in a nano-drug carrier to enhance its antimicrobial activity. Furthermore, in vitro, and in vivo wound healing studies of the novel nano-chitosan/Eucalyptus oil/cellulose acetate electrospun nanofibers were investigated. Eucalyptus oil showed a potent antimicrobial activity against the tested pathogens and the highest inhibition zone diameter, MIC, and MBC (15.3 mm, 16.0 μg/mL, and 256 μg/mL, respectively) were recorded against Staphylococcus aureus. Data indicated a three-fold increase in the antimicrobial activity of Eucalyptus oil encapsulated chitosan nanoparticle (43 mm inhibition zone diameter against S. aureus). The biosynthesized nanoparticles had a 48.26 nm particle size, 19.0 mV zeta potential, and 0.45 PDI. Electrospinning of nano-chitosan/Eucalyptus oil/cellulose acetate nanofibers was conducted, and the physico-chemical and biological properties revealed that the synthesized nanofibers were homogenous, with a thin diameter (98.0 nm) and a significantly high antimicrobial activity. The in vitro cytotoxic effect in a human normal melanocyte cell line (HFB4) proved an 80% cell viability using 1.5 mg/mL of nano-chitosan/Eucalyptus oil/cellulose acetate nanofibers. In vitro and in vivo wound healing studies revealed that nano-chitosan/Eucalyptus oil/cellulose acetate nanofibers were safe and efficiently enhanced the wound-healing process through enhancing TGF-β, type I and type III collagen production. As a conclusion, the manufactured nano-chitosan/Eucalyptus oil/cellulose acetate nanofiber showed effective potentiality for its use as a wound healing dressing.
This page summarises published work. The authoritative version sits with the publisher.
DOI: 10.3390/membranes13060604
Is something wrong with this record? Report it or request removal.
Discussion
Have you built on this work, tried to replicate it, or seen it applied in practice? Share what you know. Verified researchers and MARATTO™ domain experts can open a discussion, and any member can reply. Contributions are reviewed before they appear.
No discussion yet. Open the first thread.
New to MARATTO™? Create a free account.
