Molecular Evaluation of prognostic Markers of Breast Cancer (BC) after Treatment in vitro

Abstract

Breast cancer is a huge burden and poses serious threats to women's health worldwide.Despite significant advances in breast cancer biology and treatment methods.Usually, cancer is related to overexpression or misregulation of important genes which in turn, confer risk of developing breast cancer.Curcumin which is a natural compound found in the spice turmeric has been shown to have a number of potential health benefits, including antioxidant and anti-inflammatory effects.Recently, research has also suggested that curcumin may have potential as a treatment for breast cancer.Studies have shown that curcumin may be able to inhibit the growth of breast cancer cells and induce cell death in some types of breast cancer cells.It may also have the ability to inhibit the formation of new blood vessels through angiogenesis, which is necessary for cancer cells to grow and spread.This study is designed to investigate the effect of curcumin on of HER-2, PS2 and CTD on breast cancer.To achieve this aim we conducted the study on MCF-7 cell lines, and evaluate the cytotoxicity of different concentration of curcumin by MTT assay, RNA extracted from MCF-7 cell lines by Qiagen method and reverse transcriptase to cDNA finally quantification of the expression of genes (HER-2, PS2 and CTD) by Real time PCR (RT-PCR).The results showed that different concentration of curcumin make inactivation for genes related to breast cancer (HER-2, PS2 and CTD) and can stop the breast cancer pathway.