Isolation, Identification and Antibiotics Susceptibility Test of <i>Escherichia</i> <i/> <i>coli</i> from Abattoir and Butchers in Bishoftu Town

Abstract

E. coli with multiple antibiotics resistance is one of the public health issues concerned with the Food of animal origin safety. To control this pathogen; isolation and determining of antimicrobial resistance is a key tool. Therefore, the objective of the current study was to isolate, identify and detect the antimicrobial resistance of E. coli isolated from meat sample collected from selected slaughter house and Butchers in Bishoftu town central Ethiopia. Purposive sampling techniques were applied to collect 80 samples from which 47 Carcass swab. 12 material swabs and 21 carcasses from the selected abattoir (32) and Butchers (48). Conventional bacteriological laboratory culturing, on Eosin-Methylene blue to isolate general E. coli and on Sorbitol MacConkey agar to isolated pathogenic E. coli from general E. coli and biochemical test for E. coli identification and Disc diffusion antimicrobial susceptibility test were applied to isolate The E. coli. Out of 80 samples tested 18.75% of the samples were positive for the occurrence of the E. coli : The incidence of E. coli occurrence in two different meat value chain (Abattoir and Butcher) indicated the frequency of 25% and 14.5% respectively with no significant difference between them (P-value &gt; 0.05). For antimicrobial sensitivity test out of eleven (11) antibiotics disk selected, two drugs, namely; Ampicillin and penicillin were 100% resistant to fifteen E. coli isolates; while fifteen isolates of E. coli were 100% susceptible to Gentamycin and sulphametazole. The antibiotic resistant test result in current study indicated that each E. coli isolate was resistant to at least two antibiotics. Therefore, Good hygienic and safety practice should be applied both at slaughter house and butchers, awareness creation among the society about the risk of transmission, prevention and controls of multidrug resistant E. coli should be given.