Investigation of anti-oxidant activities of pyrazole based-compounds in a mice model of rheumatoid arthritis

Abstract

Rheumatoid arthritis (RA) is an autoimmune disease that causes joint inflammation and cartilage damage. Free radicals can harm the joint cartilage. Pyrazole compounds have variety of medical applications, including anti-inflammatory properties. Purpose of the study: The purpose of the study was to compare the effect of pyrazole derivatives on catalase, GSH, and NO levels in a mouse model of RA to indomethacin as a reference medicine in terms of oxidative stress. Basic procedures: Rheumatoid arthritis mouse model was created by immunization of DBA/1J mice with chicken type-II collagen and complete and incomplete Freund's adjuvants. Mice were placed into two groups, group I: control and group II: Rheumatoid arthritis mice: Mice were injected intradermal at the base of their tails with 100 μl chicken type II collagen emulsified in CFA and received a booster intradermal shot after three weeks of 100 μl l of chicken type II collagen emulsified in IFA. Mice with arthritis were categorised and treated as follows: Indomethacin group; treated with 1 mg/kg SC of indomethacin daily for five days. The M1E group; received M1E 5mg/kg SC daily for five days and fifteen days respectively. The M1G group received M1G 5mg/kg SC daily for five days and fifteen days respectively. Main findings: Pyrazole derivatives M1E and M1G dramatically improved catalase and GSH levels and decreased NO levels in arthritic mice. Principle conclusion: Two pyrazole derivatives, M1E and M1G, were shown to alleviate oxidative stress by increasing catalase and GSH activities and suppressing NO levels in RA mice.