Influence of Seed Soaking and Foliar Application Using Ozonated Water on Two Sweet Pepper Hybrids under Cold Stress

Abstract

The harmful impacts of ozone (O3) on plant development and productivity have been excessively studied. Furthermore, the positive influences of its low concentrations still need to be explored further. The present study was performed to assess the impact of low concentrations of O3 on two sweet pepper hybrids under cold stress. The ozonated water was utilized for seed soaking or foliar application at concentrations of 0, 10, 20, 30, and 40 ppm. Seed soaking using ozonated water for 1 h was compared to soaking in distilled water as a control. Moreover, exogenously ozonated water was sprayed thrice at three-day intervals compared with untreated control. The differences between the applied methods (seed soaking and foliar application using ozonated water) were not statistically detected in most of the evaluated parameters. On the other hand, the evaluated hybrids displayed significant differences in the studied parameters, with the superiority of the Lirica evident in most germination and seedling growth parameters. Both applied methods significantly improved germination and seedling growth parameters. In particular, the concentration of 40 ppm displayed the highest enhancement of the germination index, coefficient velocity, and seedling quality. In addition, it promoted the seedling maintenance of high relative water content (RWC), chlorophyll, proline, and ascorbate peroxidase activity under cold stress conditions. Moreover, it protected the cell wall from damage by decreasing membrane permeability (MP). Generally, the best results were obtained from 40 ppm followed by 30 ppm of O3 as seed soaking or foliar spray. The results pointed out the possible use of O3 in a low concentration to protect the plants from cold stress during germination and early plant growth.