Influence of microwave assisted extraction process para-meters on the antioxidant activity of xanthone-rich extracts from roots of Securidaca longipedunculata

Abstract

Roots of Securidaca longipedunculata have been used for their medicinal properties. These roots are rich in bioactive compounds with many pharmacological properties, such as anti-inflammatory and anti-oxidant effects. This research investigated the effects of microwave-assisted extraction parameters on the anti-oxidant activity of extract from S. longipedunculata roots. The influences of irradiation time, microwave power, and solvent-to-material ratio on the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, and the ferrous ion chelating activity, as well as total xanthones, were used to optimize extraction parameters and to investigate their interactions through Response Surface Method (RSM). The optimal combined conditions for extracting maximum xanthones (2888 μgEDMX/gP) and anti-oxidant activity (DPPH 82.24%, chelating Iron 88.28%) were irradiation time 78.34 s, microwave power 529 W, solid to liquid ratio 1.01 g/20 mL. The extract obtained at optimal conditions has a very high anti-oxidant activity compared to an extract obtained with conventional method extraction. Furthermore, Higher Performance Liquid Chromatography (HPLC) analysis of optimal conditions with MAE showed remarkable differences in peak than in traditional extraction. Scanning electron micrograph (SEM) of roots of S. longipedunculata showed morphological changes and cellular damage compared to the initial powder. The results indicate the total uzes of the extract from S. longipedunculata roots. This study design guides future efficient use of this method for other valuable species or bioactive compounds.