In vitro Investigation and GC-MS Analysis of the Chemical Constituents in the Fraction of Hexane Leaf Extract of Tapinanthus bangwensis (Engl. and K. Krause) Loranthaceae

Abstract

The exploration of medicinal plants as potential alternative therapeutic model comes from the quality content of its chemical compounds that exacerbate remarkable activity, nevertheless, there is the need for further exploit. This study investigated the in vitro antioxidant and antidiabetic potential of the fraction (HEX-ETACF or CF 2) of hexane leaf extract of Tapinanthus bangwensis, but importantly analyzed the likely chemical compounds present in the sample. The study used GC-MS (gas chromatography-mass spectroscopy) machine and spectrophotometer to elucidate the possible compounds and estimate the antioxidant and antidiabetic property respectively. The fraction had significant amount of alkaloids content, while phenolics content appeared higher than the flavonoids content The antioxidant results had the activity of lipid peroxy, 2,2-diphenyl-1-hydrazyl and nitrite radicals significantly inhibited compared to the ferric radicals. Furthermore, the result showed that the fraction inhibited α-amylase's metabolic activity higher than α-glucosidase. The Fourier transition infrared (FTIR) chromatogram had ten peaks, indicated the fraction contains complex molecules that bears carbonyl, methyl, carboxyl and aromatic groups respectively. The GC-MS chromatogram revealed twenty-one peaks, such that butyl-9-octadecenoic acid (8.01%) and nonahexacontanoic acid (0.05%) had the highest and lowest percentage areas (% area). Another GC-MS chromatogram had twenty-seven peaks, with squalene (peak 23; 37.29%) and 1,2-benzenedicarboxylic acid, buty-2-ethylhexyl ester (peak 27; 19.87%) been significantly abundant, suggested the fraction was rich in phenolics and phthalates. Our findings revealed that the compounds demonstrate beneficial effects as antioxidant and antidiabetic agents.