In Vitro Antioxidant and Free Radical Scavenging Activities of Pleurotus tuber-regium

Abstract

This study evaluated the in vitro antioxidant potential of the methanol extract of Pleurotus tuber-regium (PT) using a range of established spectrophotometric assays. These included ferric reducing antioxidant power (FRAP), nitric oxide scavenging activity, ABTS radical scavenging activity, DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity, metal ion chelating activity, and hydrogen peroxide scavenging activity. The results revealed that the extract exhibited concentration-dependent antioxidant activities across all assays. Although the scavenging effects of the extract were consistently lower than those of the standard antioxidant, gallic acid, it demonstrated notable efficacy, particularly in metal ion chelation, with maximum inhibition observed at 40 mg/ml. The extract also showed moderate reducing power and the ability to scavenge various free radicals. These findings suggest that P. tuber-regium possesses significant antioxidant properties and could serve as a promising source of natural antioxidants. Therefore, it holds potential for development into plant-based pharmaceutical or nutraceutical agents for managing oxidative stress-related disorders.