IMMUNOHISTOCHEMICAL STUDY OF INTERLEUKIN 38 IN PSORIATIC PATIENTS AND ITS CORRELATION WITH INTERLEUKIN 17A.

Abstract

Psoriasis is a common chronic inflammatory skin disease that affects 2–3% of the world’s population and greatly impairs the quality of life of affected individuals.Recent studies showed that IL36R blockade by IL-36Ra or a neutralizing IL-36R antibody decreases the inflammation in ex vivo and in vivo experimental models of psoriasis.IL-38 is a 17–18 kDa protein sharing 40% sequence similarity with IL-1RA and IL-36Ra antagonists and elicits it’s antagonistic effects through binding to IL-36 receptor, as IL-36Ra.IL-38 is reduced in the epidermis of psoriatic lesions as compared with uninvolved skin.AIM OF THE WORK:The aim of this study was to assess the correlation between immunohistochemical expression of both IL17A and IL38 in psoriatic skin and their relation to disease severity and activity.METHODS:Patients were subjected to: Sex and age recording, general history taking, psoriasis history.General dermatological examination was conducted to assess the distribution and the severity of psoriasis, which was calculated using psoriasis & severity index (PASI score).• Lesions were photographed accurately using the same camera settings. • Five mm punch biopsy was taken from lesional skin for all participants mainly from trunk and legs ,The biopsies were obtained under local anesthesia.• Samples were subjected to histopathological examination with Hematoxylin and eosin and immunohistochemical study for both IL17A and IL38.Punch biopsies sized 5 mm were also obtained from healthy volunteers or normal skin specimens obtained during plastic surgeries, subjected to histopathological examination with Hematoxylin and eosin and immunohistochemical study for IL17A and IL38 as well.