HSP70, adiponectin, leptin, pro-inflammatory cytokines and metabolic hormones of heat-stressed broilers fed herbal supplements

Abstract

Global warming undoubtedly is a serious challenge facing poultry production especially in tropical climate, which subject animals to heat-stressed conditions leading to multiple physiological alterations. An investigation was conducted to determine the role of herbal supplements on adipokines, pro-inflammatory cytokines and metabolic hormones of broilers exposed to heat stress. 200 a day-old broiler chicks were randomly allotted to standard diets; diet 1 without leaf meal (T1), diet 2 with 5% Moringa oleifera (T2), diet 3 with 5% Phyllanthus amarus (T3) and diet 4 with 5% mistletoe; Viscum album (T4) in a 49-day feed trial during the peak of thermal discomfort in Southern Nigeria, to assess hormones, adipokines and cytokines using standard procedures. Results obtained shows that triiodothyronine of birds fed phyllanthus and mistletoe were significantly (p < 0.05) higher than birds on basal diet. Corticosterone of birds fed mistletoe and phyllanthus were significantly (p < 0.05) lower than those obtained in birds on basal diet. Heat shock protein of birds fed on moringa supplements were significantly (p < 0.05) lower than those in birds on basal diet. The adiponectin of birds fed on basal diet were statistically (p < 0.05) higher than other treatments. Interleukin 6 of birds on phyllanthus and mistletoe supplements were not significantly (p > 0.05) different from birds on basal diet. Interleukin 1β of birds fed basal diet was significantly (p < 0.05) higher than birds fed moringa supplements and the significantly (p < 0.05) least values obtained in those of birds fed phyllanthus supplement. Tumor necrosis factor α of birds fed on phyllanthus and mistletoe were significantly (p < 0.05) higher than birds fed basal diet and the significantly (p < 0.05) least values were obtained in birds fed moringa supplement. In conclusion, three herbal supplements decreased the HSP 70, leptin and adiponectin of broilers exposed to heat stress and moringa supplements lower pro-inflammatory cytokine expression in heat stress birds.