Highly Specific Voltammetric Detection of Cephalexin in Tablet Formulations and Human Urine Samples Using a Poly(2,4,6-2′,4′,6′-hexanitrodiphenylamine)-Modified Glassy Carbon Electrode

Abstract

β-Lactam antibiotics are employed to treat bacterial illnesses. Despite a high level of clinical success, they have encountered serious resistance that demands a high-dose regimen and a new pharmacokinetic combination. This requires continuous monitoring of their levels in pharmaceutical and biological samples. In this study, an electrochemical sensor was developed for the determination of cephalexin (CLN) in pharmaceutical formulations and biological fluid samples. The sensors were developed by modifying a glassy carbon electrode (GCE) using a conducting polymer (dipicrylamine) by potentiodynamic electropolymerization. Characterization (using cyclic voltammetry and electron impedance spectroscopy) results revealed modification of the electrode surface, leading to an enhanced effective electrode surface area and their conductivity. The appearance of an irreversible oxidative peak at much-reduced potential with 5-fold current enhancement at a poly(dipicrylamine)-modified glassy carbon electrode (poly(DPA)/GCE) verified the electrocatalytic role toward CLN. Under optimized conditions, a wider linear concentration range (5 × 10^-8 to 3.0 × 10^-4 M), lowest limit of detection (LoD) (2.5 nM), detected amount of each tablet brand above 97.00% of the labeled value (showing excellent agreement between the detected amount and company label), and excellent % recovery results in pharmaceutical and biological samples were obtained with an excellent interference recovery error of less than 4.05%. Its excellent accuracy, selectivity, reproducibility, and stabilities and only requiring a simple electrode modification step combined with its readily available and nontoxic modifier, which sets it apart from most previously reported methods, have validated the present method's potential applicability for determining CLN in biological and pharmaceutical samples.