article · Artificial Cells Nanomedicine and Biotechnology
Silver nanoparticles can be synthesised using an eco-friendly method derived from the leaf extract of Eriobotrya japonica, commonly known as loquat. Successful formation of the nanoparticles was verified through techniques including UV-visible spectroscopy, scanning electron microscopy, zeta potential analysis, Fourier-transform infrared spectroscopy and X-ray diffraction. The resulting particles exhibited a quasi-spherical shape, negative surface charge and crystalline structure. Laboratory testing revealed notable anti-proliferative effects against MCF-7 breast cancer and HeLa cervical cancer cell lines, accompanied by apoptosis demonstrated through DNA fragmentation and P53 expression. Furthermore, the nanoparticles reduced inflammation, lessened allergic reactions and promoted phagocytosis in bone marrow-derived macrophages, suggesting multiple pathways for biomedical intervention.
Using plant extracts to fabricate metallic nanoparticles avoids toxic chemicals and supports sustainable drug discovery. Demonstrating that these green-synthesised silver particles tackle cancer cell proliferation while simultaneously calming inflammation, reducing allergic responses and boosting immune cell phagocytosis provides an encouraging basis for developing multi-target biomedical therapies from natural plant sources.
This research is at an early laboratory stage, focused on in vitro cell lines and basic immunological models. It could eventually interest pharmaceutical developers and biotechnology companies looking for green-synthesised nanomaterials to act as immune-modulating agents, vaccine adjuvants or nano-drug delivery platforms. Significant preclinical development, safety evaluation and in vivo testing will be required before any commercial or clinical application is feasible.
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Nanoparticles (NPs) have biological activities like antibacterial, antifungal, drug delivery, immunomodulation and antitumor activities. The aim of the current study was to investigate some of biomedical applications of silver NP synthesis using extracts from leaves of <i>Eriobotrya japonica</i>. Colour changes, UV-visible spectroscopy, SEM, zeta potential, dynamic light scattering, FTIR and XRD were used to confirm AgNPs formation. The UV-vis spectrum absorption band was observed at almost 430 nm. The SEM image shows quasi-spherical shape of AgNPs. The zeta potential demonstrated the negative surface charge of NPs. FTIR results showed the functional groups of AgNPs. Crystalline nature of AgNPs was confirmed by XRD pattern. MTT assay was used to study the anti-proliferative activity against MCF-7 and HeLa cells. Apoptosis was tested using a DNA-fragmentation test, and expression of P<sup>53</sup>. AgNPs inhibited the proliferation of MCF-7 and HeLa cells, and reduced inflammation. Treatment with AgNPs significantly decreased allergic disorder. AgNPs stimulated the phagocytosis process in BMDMs. The results suggested that AgNPs could be a promising therapy for future and preventing inflammation, reduce allergic disorders and prevent bacterial infection through the up-regulation of phagocytosis. Hence, future work such as developed and improved NPs as adjuvants, immune-modulating substances and nano-drug delivery system is needed.
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DOI: 10.1080/21691401.2020.1867152
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