Green HPLC method for determination of paracetamol and ibuprofen in human plasma: applications to pharmacokinetics

Abstract

Using a straightforward, sensitive and precise liquid chromatographic approach, it is now possible to concurrently measure the amounts of ibuprofen (IBU) and paracetamol (PAR) in human plasma. A µ BondapakTM C18 column (300 mm × 3.9 mm, 15-20 μm) demonstrated acceptable separation when utilizing a mobile phase of 10 mM disodium hydrogen orthophosphate solution and acetonitrile at an 80:20, v/v ratio. The elution was isocratic at room temperature and a flow rate of 1.0 milliliters per minute. The UV detector was set to monitor PAR and IS (tinidazole) for 6.5 min at 254 nm, then IBU for the next 3 min at 220 nm. PAR and IBU showed linearity across the 0.05 to 100 µg/ml concentration range. The precision of the measurements ranged from 98.5% to 105% for PAR and from 95.1% to 102.8% for IBU. The average drug recovery rate was 100% for PAR and 98.9% for IBU. This method was effectively utilized to assess samples from an actual population administered PAR and IBU (325/200 mg) for pharmacokinetic research. The technique employs green and white tools to evaluate their environmental sustainability and efficacy. The suggested strategy was implemented utilizing the Six Sigma method.