Glycoplex Promising Postbiotic for Anti-inflammatory and Wound Healing Applications

Abstract

To repair and regenerate tissue and avoid infections and problems, wound healing is an essential biological process. In recent years, there has been an increasing interest in investigating sustainable wound healing techniques. The innate and adaptive immune systems' natural reaction to infection is inflammation. Low cytotoxicity natural molecules with anti-inflammatory and wound healing properties can target the key players of inflammation, wound healing and exert beneficial skin health effects. In this study, Glycoplex raw material from polygon Life science Company assayed to determine anti-inflammatory properties using red blood cells hemolysis and membrane stability method. Scratch Assay was performed to evaluate the migratory capacity of HFB4 human skin fibroblasts cells in response to treatment with Glycoplex for wound healing application. Human skin fibroblasts cells HFB4 treated with Glycoplex for cytotoxicity assay using MTT method. Anti-inflammatory results indicated that IC₅₀ of Glycoplex at 4.19µg/mL reflects potent bioactivity even at relatively low concentrations if compared with standard indomethacin recorded IC₅₀ at 4.28µg/mL. The wound healing results demonstrate that Glycoplex treatment significantly accelerates fibroblast migration and wound closure compared to the untreated control. The Glycoplex -treated group achieved nearly 90% closure within 48 hours, compared to 66% in the control group, indicating a clear enhancement of the wound healing process. The IC₅₀ value of 196.34µg/mL obtained for the Glycoplex indicates low cytotoxic activity against human dermal fibroblasts. In conclusion, Glycoplex, a novel postbiotic-based bioactive, demonstrates potent anti-inflammatory activity comparable to indomethacin, alongside significant wound-healing benefits through accelerated fibroblast migration and low cytotoxicity toward human dermal fibroblasts. Its proven efficacy in managing mild-to-moderate inflammation, combined with its safety, photo stability, and sustainable origin, positions it as a promising alternative to conventional agents and a valuable innovation in advanced wound care strategies.