Gene Expression of Oxidative/Antioxidative Markers, VDR, CAMK4 and Ceruloplasmin in Baladi Sheep a with Minerals Deficiency

Abstract

The objective of this work was to elaborate gene expression and serum profile of oxidative/antioxidant and bone markers associated with minerals deficiency and their correlation with mineral deficiency in sheep. The study was carried out on 26 selected diseases cases out of survey on 250 sheep suffered from mineral deficiency compared to 10 apparent healthy control sheep based on clinical examination. Biochemically, there was a significant (P<0.05) decline in Ca, P, Mg, Cu, Zn, Se, Fe, TAC, GPx, SOD, CAT, OC and CP, whereas MDA level was significantly increased (P<0.05). Gene expression of SOD, CAT, GPX1, VDR, CAMK4 and ceruloplasmin genes were significantly down-regulated (P<0.05) in diseased sheep. A contrary tendency was evoked by the gene OXSR1.The variability in studied genes alongside alterations in the serum profile of investigated markers could be a reference guide for limiting the mineral deficiency in sheep through prescribing an efficient nutritional management strategy for sheep flocks that includes the essential macro- and micro aspects, and not depend just on grazing as a primary source of feed to sustain sheep health and output.