article · IET Nanobiotechnology
Fungal xylanases can be utilised to synthesise silver nanoparticles with diverse functional properties. Analysis reveals that protein molecules cap and stabilise these spherical particles, which measure between 15.21 and 77.49 nanometres. Laboratory evaluations show that the resulting nanoparticles actively inhibit bacterial and fungal growth, demonstrating strong antimicrobial performance. They also exhibit significant antioxidant action through the scavenging of DPPH and hydrogen peroxide. Beyond biological inhibition, the particles function as catalysts capable of breaking down industrial dyes, specifically degrading malachite green and methylene blue. Tests using human blood samples further reveal remarkable anticoagulant and clot-dissolving, or thrombolytic, capabilities. Overall, the findings demonstrate an enzymatic biological route to produce multi-functional silver nanoparticles suitable for catalytic and medical uses.
Using enzymes rather than harsh chemicals offers a biological route for producing functional nanomaterials. These laboratory-developed silver nanoparticles possess multiple properties in a single material, including the ability to eliminate microbes, reduce oxidative stress, dissolve blood clots, and break down toxic synthetic dyes. Such multi-functional capabilities could support improved biomedical interventions and cleaner methods for managing industrial waste.
This work points towards potential applications in biomedical therapeutics, such as clot-busting or antimicrobial agents, and environmental remediation for industrial dye wastewater treatment. Potential users include developers of wound care products, cardiovascular therapies, and industrial water treatment systems. Based strictly on the reported laboratory syntheses, dye degradation, and in vitro blood assays, this technology remains at an early stage of research and requires extensive further testing before real-world adoption.
AI-generated from the published abstract. Always read the original work before citing.
L2 (TEA) to synthesise silver nanoparticles (AgNPs). Characterisation of AgNPs was carried out using UV-Vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), and transmission electron microscopy, while their effectiveness as antimicrobial, antioxidant, catalytic, anticoagulant, and thrombolytic agents were determined. The colloidal AgNPs was brownish with surface plasmon resonance at 402.5 and 410 nm for NEA-AgNPs and TEA-AgNPs, respectively; while FTIR indicated that protein molecules were responsible for the capping and stabilisation of the nanoparticles. The spherical nanoparticles had size of 15.21-77.49 nm. The nanoparticles significantly inhibited the growth of tested bacteria (63.20-88.10%) and fungi (82.20-86.10%), and also scavenged DPPH (37.48-79.42%) and hydrogen peroxide (20.50-96.50%). In addition, the AgNPs degraded malachite green (78.97%) and methylene blue (25.30%). Furthermore, the AgNPs displayed excellent anticoagulant and thrombolytic activities using human blood. This study has demonstrated the potential of xylanases to synthesise AgNPs which is to the best of our knowledge the first record of such. The present study underscores the relevance of xylanases in nanobiotechnology.
This page summarises published work. The authoritative version sits with the publisher.
DOI: 10.1049/iet-nbt.2017.0299
Is something wrong with this record? Report it or request removal.
Discussion
Have you built on this work, tried to replicate it, or seen it applied in practice? Share what you know. Verified researchers and MARATTO™ domain experts can open a discussion, and any member can reply. Contributions are reviewed before they appear.
No discussion yet. Open the first thread.
New to MARATTO™? Create a free account.
