Exploring the impact of the innovative compound 3-(3-(4-hydroxy-2-oxo-2H-chromen-3-yl)-5-(pyridin-3-yl)-1H-pyrazol-1-yl) indolin-2-one on accelerating wound recovery

Abstract

Wound healing represents a significant challenge within the field of medical science. Contemporary clinical practices increasingly favor the utilization of herbal compounds to facilitate the repair process. Among these compounds, coumarin-a phytochemical noted for its antibacterial and wound-healing properties-has garnered considerable attention. This study investigates the potential advantages of incorporating coumarin into wound dressings within an experimental model. Our synthesized target compound 3-(3-(4-hydroxy-2-oxo-2H-chromen-3-yl)-5-(pyridin-3-yl)-1H-pyrazol-1-yl) indolin-2-one (CPPI) demonstrated notable antimicrobial activity against the pathogenic Staphylococcus aureus MRSA, Bacillus cereus, and Pseudomonas aeruginosa. Moreover, both in vitro and in vivo experiments showed that CPPI significantly enhanced the migration of skin fibroblast cells and promoted the wound healing process. Furthermore, it facilitated complete re-epithelialization of the wounds. Histological analysis revealed the formation of well-structured granulation tissue and a reduction in indicators of wound infection, evidenced by a minimal presence of inflammatory cells in comparison to untreated wounds. Additionally, in silico molecular docking studies of CPPI indicated significant binding affinity within COX-2 active site along with a stable complex during molecular dynamics simulations. Collectively, the findings of this study suggest that CPPI can provide a protective effect against infections in cutaneous wounds, attributable to its antimicrobial properties.