Evaluation of Analgesic Property of Silver Nanoparticles Synthesized Using <i>Mitracarpus scaber </i>Aqueous Extract

Abstract

Pain is one of the main causes for which people seek medical attention. Strong, long-lasting, safe analgesics are therefore still needed in the medical industry to treat a wide range of painful disorders, despite recent advancements in pain treatment. The current work is intended to assess the analgesic properties of silver nanoparticles (AgNPs) synthesized using Mitracarpus scaber aqueous extract. Aqueous extraction of Mitracarpus scaber whole plant was done and employed to make and cap AgNPs using 0.1M AgNO 3 solution. The produced AgNPs were examined using a variety of methods, such as Fourier Transform Infrared Spectroscopy (FTIR), UV-VIS, and Transmission Electron Microscopy (TEM). Acetic acid writhing and hot plate tests were then used to investigate the analgesic activity of various doses of the biosynthesized AgNPs. AgNPs production was detected by UV-vis spectroscopy at λ max = 425 nm. The biosynthesized AgNPs had a size range of 4-5 nm, were crystalline and spherical in shape, according to TEM investigations. FTIR analysis on the other hand revealed the involvement of phenyl, hydroxyl, carbonyl, and aliphatic carbon-hydrogen bonds in the AgNPs' production. The biogenic silver nanoparticles showed the highest reduction of writhes and pain at the doses of 100 and 50 mg/kg b.w. respectively, which were significantly higher than the analgesic activity elicited by Diclofenac (50 mg/kg b.w.) for both assays. The present work therefore concludes that biogenic silver nanoparticles using aqueous extract of Mitracarpus scaber have strong analgesic properties and could be utilized for the management of pain after proper pharmacological evaluations.