Empagliflozin mitigated cisplatin induced renal endothelial dysfunction in rats via repressing oxidative, inflammatory, apoptotic and fibrotic signals

Abstract

Cisplatin (Cis) is an extensively prescribed chemotherapeutic agent inducing acute kidney injury (AKI) and nephrotoxicity. This study explored the possible nephroprotective effects of the antidiabetic sodium-glucose cotransporter 2 (SGLT2) inhibitor empagliflozin (Empa) against Cis-induced endothelial dysfunction and elaborated its underlying protective mechanisms. Empa (5–60 mg/kg, orally) was administered to male rats for 14 days and nephrotoxicity was induced by a single intraperitoneal injection of Cis (5 mg/kg, i.p.) at day 11 of Empa administration. Empa nullified Cis induced rise in plasma urea and creatinine. On isolated perfused kidney, Empa: a) restored phenylephrine (0.41–900 ng) intensified renal vasoconstriction, b) reinstated renovascular responsiveness to endothelium-dependent vasodilation evoked by acetylcholine (0.01–2.43 nmol), c): restored the Emax and EC50 values of ACh dose-response curves, such effects were abolished following coadministration of L -NAME, d) unchanged the vasodilatory effect of sodium nitorprusside (0.001–10 μmoles) after treatment with Cis. alone or combined with L -NAME. Empa decreased renal p-eNOS, p-eNOS/t-NOS ratio and ET-1 levels in western blot analysis and reduced plasma Nox level. Empa ameliorated renal oxidative stress (lowered reduced glutathione and increased malondialdehyde levels), reduced plasma cytokine levels (IL-1β, TNF-α), and plasma apoptotic markers (Bax and caspase 3) induced by Cis. Furthermore, immunohistochemical studies showed that Empa mitigated Cis-induced increase in the renal expression of MAPK p38 , TGF-β and SMAD3 and histologically, attenuated Cis induced glomerular and endothelial necrosis and dilatation of the renal tubular lumen. Collectively, Empa effectively mitigated endothelial dysfunction and nephrotoxic effects caused by Cis through amending oxidative stress, inflammation, apoptosis and renal fibrosis. • Empagliflozin dose dependently mitigated the signs of Cisplatin-induced AKI. • Empagliflozin blunted the vasoconstriction induced by Cisplatin in perfused kidneys. • Empagliflozin alleviated the attenuated endothelium-dependent renal vasodilatory action of ACh. • Targeting MAPK p38/TGF-β/Smad3 signaling prompts empagliflozin renoprotection in cisplatin induced endothelial dysfunction. • Empagliflozin reduced endothelial dysfunction induced by Cisplatin by amending oxidative stress, inflammation, fibrosis and apoptosis.