Electrospun Poly-Lactic Acid Nanofibers for Dual Drug Delivery in Regenerative endodontics: Ciprofloxacin and Metronidazole Loading and Characterization

Abstract

Aim: This study presents the synthesis, material characterization, and evaluation of the antimicrobial activity of antibiotic-loaded scaffolds. Materials and methods: Metronidazole (MID) or Ciprofloxacin (CIP) was incorporated into a polylactic acid (PLA) polymer solution at a concentration of 25 wt.% and then processed into Fibers. Neat PLA nanofibers were used as controls. Characterization techniques, including scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), degradation tests, and drug release assessments, were employed. The antimicrobial properties were tested against Porphyromonasgingivalis (Pg) and Enterococcus faecalis (Ef). Results: SEM imaging showed Fibers with submicron diameters, and FTIR confirmed the successful incorporation of the antibiotics. Data analysis demonstrated a gradual and sustained release of the drugs from the scaffolds over 48 hours. Antimicrobial activity was confirmed via agar diffusion assays, showing efficacy against the tested bacteria. Conclusion: Antibiotic-loaded electrospun PLA scaffolds could serve as biologically safe antimicrobial drug delivery systems for use in regenerative endodontics.