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Effects of Dietary Xylanase and Arabinofuranosidase Combination on the Growth Performance, Lipid Peroxidation, Blood Constituents, and Immune Response of Broilers Fed Low-Energy Diets

201948 citationsOpen accessKafr el-Sheikh University

In plain language

This study evaluated the effects of adding a combination of xylanase and arabinofuranosidase enzymes to low-energy diets fed to broiler chickens. Researchers tested 480 one-day-old Ross 308 chicks across three groups: a standard control diet, a reduced-energy diet, and a reduced-energy diet supplemented with the enzyme mixture. Broilers given the enzyme-supplemented low-energy feed demonstrated enhanced feed efficiency alongside improved digestibility of both protein and fat. The enzyme combination also increased total lipid content in muscle tissue while decreasing markers of lipid peroxidation. Furthermore, birds receiving the supplemented feed displayed higher antibody titres against Newcastle disease virus, as well as increased expression of genes associated with growth and fatty acid synthesis. Overall, the multi-enzyme additive mitigated the nutritional limits of low-energy formulations and supported broiler health and performance.

Key takeaways

  • Supplementing low-energy broiler feed with xylanase and arabinofuranosidase improved protein and fat digestibility.
  • The enzyme combination enhanced feed efficiency and increased total muscle lipid content while reducing lipid peroxidation.
  • Birds receiving the enzymes showed higher antibody titres against Newcastle disease virus.
  • Dietary enzyme addition up-regulated gene expression linked to growth and fatty acid synthesis.

Why it matters

Feed represents a major cost in poultry farming, and reducing dietary energy can lower input expenses but often harms bird growth. Demonstrating that specific enzyme combinations can recover feed efficiency, nutrient digestibility, and disease resistance in broilers fed lower-calorie rations offers producers practical ways to maintain flock performance and bird health while managing feed formulation costs.

Commercialisation angle

The findings are relevant to animal feed manufacturers, poultry nutritionists, and industrial broiler producers seeking to optimise lower-cost feed formulations. Because the study utilised a commercially available feed additive (Rovabio Advance) in an in vivo poultry trial, this applied research demonstrates near-market utility for immediate dietary optimisation in commercial poultry farming operations.

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Abstract

The present study was conducted to examine that impact of dietary xylanase (Xyl) and arabinofuranosidase (Abf) supplementation on the performance, protein and fat digestibility, the lipid peroxidation, the plasma biochemical traits, and the immune response of broilers. A total of 480, un-sexed, and one-day-old broilers (Ross 308) were randomly divided into three treatments with eight replicates, where chicks in the first treatment were fed basal diets and served as the control, chicks in the second treatment were fed diets formulated with reductions of 90 kcal/kg, and chicks in the third treatment were fed the same formulated diets used in the second group as well as the Xyl and Abf combination (Rovabio<sup>®</sup> Advance). Feed intake was decreased by the low energy diet, leading to an enhancement in feed efficiency enzyme supplementation in the low energy diet (<i>p</i> < 0.015). Both protein and fat digestibility were improved (<i>p</i> < 0.047) due to enzyme supplementation. Moreover, enzyme supplementation increased muscle total lipids content and decreased muscle thiobarbituric acid retroactive substance content. Furthermore, diets supplemented with Xyl and Abf exhibited an increase in antibody titers against the Newcastle disease virus (<i>p</i> < 0.026). In addition, enzyme supplementation increased gene expression related to growth and gene expression related to fatty acid synthesis. It could be concluded that dietary Xyl and Abf supplementation had beneficial impacts on growth, nutrient digestibility, lipid peroxidation, immune response, and gene expressions related to growth and fatty acid synthesis in broiler chickens fed low-energy diets.

Research topics

  • Animal Nutrition and Physiology
  • Moringa oleifera research and applications
  • Rabbits: Nutrition, Reproduction, Health

Sustainable Development Goals

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DOI: 10.3390/ani9070467

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