Effect of Morin on fluoxetine (Prozac)-induced structural changes of mice skeletal muscle: light and electron microscopic study

Abstract

Introduction: Fluoxetine (Prozac) is an antidepressant drug with an adverse effect on the tissues with its prolonged use. Morin is natural polyphenol with different health benefits due to its antioxidant, and anti-inflammatory actions. Aim: this work designed to evaluate the effect of morin on fluoxetine (Prozac)-induced structural changes of mice skeletal muscle by means of light and electron microscopic study. Materials &methods: 35 mice (50-70 gm) were used for 3 weeks and divided into 6 groups (5 rats each); Group 1: Control, Group 2: Morin low dose (MLD): mice received low dose of morin 30 mg/kg/day orally dissolved in distilled water, Group 3: Morin high dose (MHD): mice received high dose of morin 100 mg/kg/day orally dissolved in distilled water, Group 4: Prozac group (PR): mice received an intraperitoneal injection of 0.06 mg PR/mice/day in normal sterile saline. Group 5: Prozac +Morin low dose: (PR+MLD): mice that received an intraperitoneal injection of 0.06 mg PR/mice/day in normal sterile saline preceded by one hour with a low dose of morin (30 mg/kg/day) dissolved in distilled water orally, and Group 6: Prozac +Morin high dose: (PR+MHD): mice that received an intraperitoneal injection of 0.06 mg PR/mice/day in normal sterile saline preceded by one hour with a high dose of morin (100 mg/kg/day) dissolved in distilled water orally. Oral doses were by an intragastric tube. Results: PR group showed vacuolations, cellular infiltrations, small, dark central nuclei at H&E sections, decreased PAS +ve reaction, significantly increased collagen area percentage, significantly increased Cytochrome-C area percentage, myofibrils with wide separation, accumulations of mitochondria of different sizes and shapes, degenerated nucleolar membrane and vacuolated nucleoplasm at electron microscope . On the other hand, these previous changes were ameliorated by morin dose dependently. Conclusion: Morin dose dependently could protect against fluoxetine induced skeletal muscle degenerative changes in mice.