Effect of Aflasafe® Application on the Productivity, Grain Quality, and Aflatoxin Reduction in Maize (Zea mays L.)

Abstract

Aflatoxin contamination remains a major threat to food safety, crop quality, and public health in sub-Saharan Africa. Aflasafe, a biocontrol product containing atoxigenic Aspergillus flavus strains, has proven effective in reducing aflatoxin levels, yet its broader agronomic impacts are less understood. This study evaluated the effects of Aflasafe applied at vegetative and flowering stages on maize growth, yield, nutritional composition, and postharvest quality. Application at the flowering stage led to marked improvements in vegetative growth, including plant height, leaf area, and stem girth. Yield components such as ear number, grain count, and overall yield were also significantly enhanced. Proximate composition analysis indicated elevated levels of crude fat, protein, and carbohydrate contents. In addition, grains treated with Aflasafe showed higher germination rates and seed vigor, together with reduced populations of toxigenic A. flavus and aflatoxin concentrations that remained within internationally accepted safety limits.These results demonstrate Aflasafe’s dual potential as a biocontrol and growth-promoting agent, advancing sustainable maize production, food security, and public health resilience. Keywords: