Diversity of soil bacterial communities in response to fonio (Digitaria exilis Stapf) genotypes and pedoclimatic conditions in Benin

Abstract

In sub-Saharan regions, soil fertility is a major concern for plant productivity, influenced by physical, chemical, and biological components. Among biological properties, the recruitment of soil microbial communities by plant roots is influenced by both physico-chemical soil properties and plant characteristics, dependent on species or genotypes. Here, rhizosphere bacterial communities associated with five fonio genotypes cultivated under three pedoclimatic conditions were investigated. Rhizosphere soils were collected for high-throughput 16S rRNA gene sequencing to characterize soil bacterial diversity. Additional parameters were assessed to classify soil fertility of the three pedoclimatic conditions and to evaluate relationships between the bacterial community's composition and soil fertility variables. Principal Component Analysis revealed a clear effect of pedoclimatic condition, whereas genotype had no significant impact on soil chemical properties or enzyme activities. Overall, soils were low in fertility, with Boukoumbe soil standing out for its higher chemical values and enzyme activities. For example, Boukoumbe reached 1.48% organic carbon, compared to 0.61% in Gogounou and 0.36% in Ina. Similarly, total nitrogen and available phosphorus were also higher in Boukoumbe. Regarding bacterial community, there is no impact of pedoclimatic condition and genotype on their richness and diversity. However, Bray-Curtis index revealed a significant difference in bacterial community structure among pedoclimatic conditions, but not among fonio genotypes. This suggests, in rhizosphere, bacterial community structure is more modulated by soil properties than crop genotypes. Proteobacteria and Bacteroidota were most abundant phyla, varying significantly across pedoclimatic conditions. Moraxellaceae and Oxalobacteraceae bacteria were most abundant families within Proteobacteria, while Chitinophagaceae and Weeksellaceae dominated in Bacteroidota. Our study highlighted the significant roles of soil pH, as well as sulfate and nitrate content, in shaping bacterial communities. These findings offer valuable insights into the bacterial communities associated with fonio and their key drivers.