Cytotoxicity, Antioxidant and Anti-inflammatory activities of Bis- ketone 1,3-Bis (2-acetylphenoxy)-2-propanol: An In silico, In vitro and In vivo studies

Abstract

In this investigation, bis-ketone 1,3-Bis(2-acetylphenoxy)-2-propanol (Bis-AcPh) was synthetized by Willamson ether reaction via the condensation between 1,3-dichloropropanol and 2’hydroxyacetophenone. Structural elucidation of the product was established using different spectroscopic models IR, UV, as well as 1H-NMR and 13C-NMR. This compound was screened for their antioxidant, cytotoxicity, and both in vitro and in vivo anti-inflammatory activities, and through the density functional theory some theoretical studies were carried out. The antioxidant effect was assessed by performing DPPH radical scavenging, and reducing power method. In vitro anti-inflammatory activity was estimated using the egg albumin denaturation test, while the topical anti-inflammatory effect was investigated through xylene and croton oil-induced ear oedema in mice model. Results demonstrate that the molecule exhibited moderate scavenging activity than the references antioxidants BHT and ascorbic acid, whereas it displayed a potent in vitro anti-inflammatory activity at the concentration of 2.5 mg/mL with values of 95%. In topical anti-inflammatory activity, the compound exhibited an excellent anti-inflammatory in xylene as well as in croton oil-induced ear edema in mice with a percentage of 86.15 % and 71.28%, respectively. Finally, noteworthy hemolytic degree towards human electrolytes (RBCs) was less than 2.00 %.