Cytospora: an important genus of canker pathogens

Abstract

<i>Cytospora</i> species have commonly been reported as important plant pathogenic fungi with wide host ranges and geographic distributions. With the increase in the number of cryptic species being described, a comprehensive global taxonomic revision of the genus <i>Cytospora</i> is required. The present study includes 399 isolates from 32 countries. These isolates were subjected to DNA sequence analysis for five genomic loci (ITS, <i>act1</i>, <i>rpb2</i>, <i>tef1-α</i> and <i>tub2</i>). Based on these data, it could be confirmed that <i>Cytospora</i>, <i>Leucostoma</i>, <i>Valsa</i>, <i>Valsella</i> and <i>Valseutypella</i> are congeneric. Furthermore, 111 species of <i>Cytospora</i> could also be reassessed, 44 species and four combinations newly introduced, and new typifications proposed for a further three species. Three asexual morphological groups (including 13 asexual morphological types) and three sexual morphological groups (including eight sexual morphological types) were designated. The present study explored the species diversity of <i>Cytospora</i> and re-evaluated the identity of all cultures in the Westerdijk Fungal Biodiversity Institute (Utrecht, The Netherlands) that were deposited as either <i>Cytospora</i> or as one of its related genera. This is the most comprehensive phylogenetic analysis thus far conducted on <i>Cytospora</i> and the results contribute to an increased understanding of the taxonomy of these important fungi. It is also hoped that the findings will lead to improved management strategies for diseases associated <i>Cytospora</i> species. <b>Taxonomic novelties: New species:</b> <i>Cytospora acericola</i> X.L. Fan & C.M. Tian, <i>C. adamsii</i> Jami, Crous & M.J. Wingf., <i>C. beijingensis</i> L. Lin & X.L. Fan, <i>C. betulae</i> Jami, Crous & M.J. Wingf., <i>C. brabeji</i> Jami, Crous & M.J. Wingf., <i>C. castaneicola</i> L. Lin & X.L. Fan, <i>C. cerebriformis</i> L. Lin & X.L. Fan, <i>C. conceptaculata</i> L. Lin & X.L. Fan, <i>C. crataegina</i> X.L. Fan & C.M. Tian, <i>C. deqinensis</i> L. Lin & X.L. Fan, <i>C. diqingensis</i> L. Lin & X.L. Fan, <i>C. eastringensis</i> L. Lin & X.L. Fan, <i>C. elaeagnina</i> L. Lin & X.L. Fan, <i>C. fraxinea</i> X.L. Fan & C.M. Tian, <i>C. guyuanensis</i> L. Lin & X.L. Fan, <i>C. jiufengensis</i> L. Lin & X.L. Fan, <i>C. lauricola</i> L. Lin & X.L. Fan, <i>C. lhasaensis</i> L. Lin & X.L. Fan, <i>C. lijiangensis</i> L. Lin & X.L. Fan, <i>C. lvxinensis</i> L. Lin & X.L. Fan, <i>C. malvicolor</i> X.L. Fan & C.M. Tian, <i>C. multiseriata</i> L. Lin & X.L. Fan, <i>C. nanyangensis</i> X.L. Fan & C.M. Tian, <i>C. polyspora</i> X.L. Fan & C.M. Tian, <i>C. pseudochrysosperma</i> L. Lin & X.L. Fan, <i>C. qinghaiensis</i> L. Lin & X.L. Fan, <i>C. qingshuiensis</i> L. Lin & X.L. Fan, <i>C. sanbaensis</i> L. Lin & X.L. Fan, <i>C. shaanxiensis</i> L. Lin & X.L. Fan, <i>C. shangrilaensis</i> L. Lin & X.L. Fan, <i>C. sidaohensis</i> L. Lin & X.L. Fan, <i>C. sinensis</i> L. Lin & X.L. Fan, <i>C. songshanensis</i> L. Lin & X.L. Fan, <i>C. suecica</i> Jami, Crous & M.J. Wingf., <i>C. syringina</i> L. Lin & X.L. Fan, <i>C. tenebrica</i> L. Lin & X.L. Fan, <i>C. tetraspora</i> L. Lin & X.L. Fan, <i>C. tongzhouensis</i> X.L. Fan & C.M. Tian, <i>C. uniloculata</i> L. Lin & X.L. Fan, <i>C. washingtonensis</i> Jami, Crous & M.J. Wingf., <i>C. xiaolongmenensis</i> L. Lin & X.L. Fan, <i>C. yinchuanensis</i> L. Lin & X.L. Fan, <i>C. yuduensis</i> L. Lin & X.L. Fan, <i>C. yulinensis</i> L. Lin & X.L. Fan. <b>New combinations:</b> <i>Cytospora auerswaldii</i> (Nitschke) L. Lin & X.L. Fan, <i>C. multicollis</i> (Checa <i>et al</i>.) L. Lin, X.L. Fan & Crous, <i>C. tristicha</i> (De Not.) L. Lin, X.L. Fan & Crous, <i>C. weiriana</i> (Petr.) X.L. Fan & Crous. <b>New replacement names:</b> <i>Cytospora desmazieri</i> L. Lin, X.L. Fan & Crous, <i>C. fuckeliana</i> L. Lin, X.L. Fan & Crous, <i>C. hoffmannii</i> L. Lin, X.L. Fan & Crous, <i>C. massarii</i> L. Lin, X.L. Fan & Crous, <i>C. nitschkeana</i> L. Lin, X.L. Fan & Crous, <i>C. saccardoi</i> L. Lin, X.L. Fan & Crous. <b>New synonyms:</b> <i>Cytospora ampulliformis</i> Norph., Bulgakov, T.C. Wen & K.D. Hyde, <i>C. brevispora</i> (G.C. Adams & Jol. Roux) G.C. Adams & Rossman, <i>C. cenisia</i> Sacc., <i>C. ceratospermopsis</i> C.M. Tian & X.L. Fan, <i>C. cotini</i> Norph., Bulgakov & K.D. Hyde, <i>C. ershadii</i> Zafari & Hanifeh, <i>C. erumpens</i> Norph., Bulgakov, T.C. Wen & K.D. Hyde, <i>C. fraxinigena</i> Senan., Camporesi & K.D. Hyde, <i>C. galegicola</i> Q.J. Shang, E. Camporesi & K.D. Hyde, <i>C. granati</i> D.P. Lawr., L.A. Holland & Trouillas, <i>C. hippophaicola</i> Spetik, Eichmeier, Gramaje, Stuskova & Berraf-Tebbal, <i>C. massariana</i> Sacc., <i>C. nivea</i> (Hoffm.) Sacc., <i>C. parakantschavelii</i> Norph., Bulgakov, T.C. Wen & K.D. Hyde, <i>C. parasitica</i> Norph., Bulgakov & K.D. Hyde, <i>C. paratranslucens</i> Norph., Bulgakov, T.C. Wen & K.D. Hyde, <i>C. pini</i> Desm., <i>C. populicola</i> D.P. Lawr., L.A. Holland & Trouillas, <i>C. predappioensis</i> Q.J. Shang, Norph., Camporesi & K.D. Hyde, <i>C. quercicola</i> Senan., Camporesi & K.D. Hyde, <i>C. rosae</i> Senan., Camporesi & K.D. Hyde, <i>C. salicella</i> Sacc., <i>C. vinacea</i> D.P. Lawr., Travadon & Pouzoulet, <i>Valsa germanica</i> Nitschke, <i>V. massariana</i> De Not., <i>V. nivea</i> (Hoffm.) Fr., <i>Valsella salicis</i> Fuckel, <i>Sphaeria nivea</i> Hoffm. <b>Typification: Lecto- and epitypifications (basionyms):</b> <i>Sphaeria chrysosperma</i> Pers., <i>Valsa eucalypti</i> Cooke & Harkn., <i>Valsella salicis</i> Fuckel. <b>Citation:</b> Lin L, Fan XL, Groenewald JZ, Jami F, Wingfield MJ, Voglmayr H, Jaklitsch W, Castlebury LA, Tian CM, Crous PW (2024). <i>Cytospora</i>: an important genus of canker pathogens. <i>Studies in Mycology</i> <b>109</b>: 323-401. doi: 10.3114/sim.2024.109.05.