Curative effect of zucchini flower extract on renal toxicity in male rats

Abstract

Zucchini (Cucurbita pepo L.) flowers considered as agriculture wastes. Even though its contain high different quantities of nutrients and bioactive components. There isn't much information on the possible nutritional and healthy benefits of zucchini flowers. So, the aim of the current investigation study was to determine zucchini flower extract (ZFE) might had the curative effect of treating renal injured hyperuricemic rats. As well as studying the impact of ZFE on liver and kidney as anticancer. 24 male albino rats were divided into four groups (n = 6) for this study. The first group functioned as the negative control and just received a basic diet. The remaining rats (n = 18) were given a basic diet and injected with gentamicin (100 mg/kg BW/day) for 7 days to cause hyperuricemia, which results in kidney injury. The hyperuricemic rats were subsequently split into three groups; the first group acted as the positive control and did not get any therapy, while the second and third groups each received an oral dose of the solution contained 250 and 500 mg/kg ZFE for 30 days. Interestingly, the findings revealed that the hyperuricemic group receiving a meal supplemented with 500 mg/kg ZFE did not differ substantially from the negative control in the biological parameters assessed and saw a considerable improvement in renal function compared to the hyperuricemic rats (positive control). When compared to rats with hyperuricemia (positive control), the group of hyperuricemic rats given 500 mg/kg ZFE had significantly lower levels of cholesterol, low-density lipoprotein, triglycerides, malondialdehyde, urea, creatinine, AST and ALT, with higher levels of total protein (p ≤ 0.05). The biologically favorable action of ZFE may be ascribed to its possible increase of antioxidant status through enhancement of glutathione peroxidase and catalase activities. In HepG-2 cells (which represent human hepatocellular carcinoma) and Vero cells (which represent kidney epithelium), ZFE also shown a considerable cytotoxicity. These findings might serve as an experimental foundation for further study into the possible anti-hyperuricemic and anticancer effects of ZFE.