Comparison of fine-scale malaria strata derived from population survey data collected using RDTs, microscopy and qPCR in South-Eastern Tanzania

Abstract

This study highlights significant fine-scale variability in malaria burden within and between the study districts and emphasizes the variable performance of the testing methods when stratifying risk at local scales. While RDTs and microscopy were effective in high-transmission areas, they performed poorly in low-transmission settings; and classified most villages as very low or low risk. In contrast, qPCR classified most villages as moderate or high risk. The findings emphasize that, where precise mapping and effective targeting of malaria are required in localized settings, tests must be both operationally feasible and highly sensitive. Furthermore, when planning microstratification efforts to guide local control measures, it is crucial to carefully consider both the strengths and limitations of the available data and the testing methods employed.